Lars Kristensen

Specialty Quantitative clinical proteomics. Analysis of proteins from human cell lines or clinical samples such as plasma or tissue using mass spectrometry. Quantitative techniques based on stable isotope labeling by amino acids in cell culture (SILAC), isobaric tag for relative and absolute quantification (iTRAQ), dimethyl labeling, and selected reaction monitoring (SRM). Metholoogy experience Mass spectrometry: Sample preparation. MALDI MS. Nano ESI and LC-ESI MS/MS analysis on QSTAR (QqTOF), LTQ-FT (FTICR), LTQ-Orbitrap, and TSQ (QQQ) instruments. Intact mass analysis on QTOF. Phospho-proteomics. Quantitative proteomics analysis using Stable Isotope Labeling by Amino acids in Cell culture (SILAC), Isobaric Tag for Relative and Absolute Quantification (iTRAQ), dimethyl labeling, and peptide quantification by Selected Reaction Monitoring (SRM). Large scale data processing and bioinformatics analysis. Biochemistry: Protein purification by SEC, RP, HILIC, IEC, and affinity chromatography using custom columns or HPLC. Protein kinase assays. Immunoprecipitation. Western Blotting. Fmoc peptide synthesis. Cell biology: Culture and transfection of mammalian cell lines. Culture and differentiation of human mesenchymal stem cells. Virus production and infection of insect cell lines. Cell cycle and proliferation analysis by flow cytometry. Fluorescence- and confocal microscopy. Molecular biology: Real-time RT-PCR. Gene cloning e.g. Gene sequencing, transformation, mutagenesis, and expression of recombinant protein in bacteria. Extracurricular experience Public relations consultant for Fyns Tennis Union. Responsible for writing press releases, optimizing external communication and visibility in the media (since 2012). Chairman of Stjernen Tennis. Responsible for management, communication, and marketing (since 2010). Committee member in Stjernen Tennis. Responsible for coaching of adults, webmaster of homepage, online booking system and Facebook fan page (since 2005)